Lab

Determining the Affect of Subst mark Concentration on the mark Rate of Reaction for a Catalase Extract (from Beef Liver) in a 3 Minute Period Introduction: In this investigation, it go away be determined whether the changing subst enjoy preoccupation (through dilution of 3% hydrogen bleach) has an effect on the swan reception with catalase evoke (of beef liver). The substrate that volition be utilize is hydrogen peroxide (H2O2). An enzyme which accelerates the breakdown of hydrogen peroxide into water and atomic number 8 gas is cognize as a catalase. 2H2O2--------catalase--------------> 2H2O + O2 A agency which decreases the activation vital force of a chemical reception and ontogeny the rate of chemical answer is a catalyst. So a catalase is an organic fertilizer catalyst. In this experiment, hydrogen peroxide will be used as the substrate for the catalase. Enzyme activity is proportionally to substrate soaki ng up at low substrate concentration. At higher substrate concentration there are more collisions involving the substrate and nimble site. Since the active sites are all taken up in high substrate concentration for the enzyme, when the substrate concentration is addition there is no effect.

The graph below shows the change in the rate of reaction with higher substrate concentration, where the rate of reaction reaches a peak then is leveled off: Source: hypertext counterchange protocol://www.rsc.org/education/teachers/learnnet/cfb/enzymes.htm In this experiment, the substrate concentration will be man ipulated by diluting the 3% hydrogen peroxid! e. Furthermore, the initial reaction rate with the catalase extract (beef liver) will be determined by the atomic number 8 output in intervals of 20 seconds for a period of 3 minutes (180 seconds). Research wonder: What is the affect of the change in substrate concentration (dilution of 3% hydrogen peroxide) on the rate of rate with catalase extract (beef liver)? possible action: As the substrate concentration (of...If you want to get a full essay, order it on our website: BestEssayCheap.com

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